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KMID : 1134820080370010124
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Journal of the Korean Society of Food Science and Nutrition
2008 Volume.37 No. 1 p.124 ~ p.128
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Composition and Quantitative Analysis of Stilbenoids in Mulberry (Morus alba L.) Leaves and Fruits with DAD/UV HPLC
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Kim Ji-Sun
Ha Tae-Youl
Ahn Ji-Yun
Kim Hyun-Ku
Kim Sun-A
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Abstract
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This study was aimed to analyze the composition and to quantify the contents of stilbenoids in the leaves and fruits of Morus alba L. using high performance liquid chromatography with phodtodiode array detector and UV detector. Optimal wavelength for the detection of various stilbenoids such as resveratrol, piceatannol, rhapontigenin, astringin, pterostilbene, piceid, rhaponticin and vitisin A was screened by DAD detector and set to 308 nm. Seven kinds of stilbenoids except vitisin A were identified in fruits, while 5 kinds of stilbenoids in leaves. Total stilbenoids contents were 609.15±7.24 mg/100 g d.w. in fruits and 188.57±1.70 mg/100 g d.w in leaves. Stilbenoids contents in fruits were 3 times higher than those in leaves. Rhaponticin was the most profound stilbene, analyzed to 389.26±5.22 mg/100 g d.w. (63.8% of total stilbenoids) in fruits and 99.17±2.79 mg/100 g d.w. (52.5% of total stilbenoids) in leaves. Astringin and piceatannol were only detected in fruits
and vitisin A was not detected. Contents of piceid and rhaponticin were higher than those of aglycone forms, rhapontigenin and resveratrol.
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KEYWORD
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Morus alba L., mulberry fruit, mulberry leaves, rhaponticin, photodiode array detector
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